A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two more splice variants had been claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best final results are...
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This guidebook outlines even more achievable optimization of cellular and immunoassay parameters to make sure the best possible results are...
Antibodies bound to the blocking peptide now not bind on the epitope within the concentrate on protein. By comparing the staining from your blocked antibody vs . the antibody by yourself, you can see which staining is specific.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation designs which lead towards the temporal coordination of every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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